New simple isocratic analytical method has been developed and validated for estimation of Ranitidine and Ondansetron simultaneously by RP-HPLC in bulk and pharmaceutical combined dosage form. The mobile phase was pumped at a flow rate of 1.0ml/min and UV detection was found at 225nm. Here two drugs available in Ranitidine (Rt = 2.514 min) and Ondansetron (Rt = 6.354 min) having a good resolution. The developed method was validated as per ICH guidelines. The developed method was validated for precision, accuracy, sensitivity, robustness and ruggedness.

Patients with diabetes mellitus are more likely to suffer from bone fractures. Eucommia ulmoides has previously been shown to be a reducer in osteoporosis. However, it is yet unknown if Eucommia ulmoides can prevent osteoporosis in diabetic rats. The effect of Eucommia ulmoides on bone oxidative stress and turnover indicators in diabetic rats is investigated in this study. Diabetic is induced by streptozotocin (STZ). For 8 weeks, diabetic Sprague-Dawley rats (n = 6) were given one of three treatments via gavage: Saline (control), metformin (1000mg/kg bwt), or methanol extract of Eucommia ulmoides (500mg/kg bw). As a normal control group, a group of healthy rats was used. ELISA assays were used to evaluate serum levels of insulin, oxidative stress and bone turnover indicators. When diabetic rats were given Eucommia ulmoides, insulin and osteocalcin levels were considerably higher than in diabetic control rats. By increasing osteogenesis and decreasing bone oxidative stress, Eucommia ulmoides may be able to prevent diabetic osteoporosis. These data support the use of Eucommia ulmoides as an osteoporosis treatment in diabetic patients.

The Cucumis sativus fruit (CSLJ) was subjected to hydrogen peroxide radical scavenging assay in reference to butylated hydroxytoluene. This study was carried out to evaluate the anti-inflammatory and antioxidant activities of Cucumis sativus Linn. (Family: Cucurbitaceae) leaves. The methanolic extract of Cucumis sativus leaves (MCS) were  investigated for anti-inflammatory activities at different doses of 150 and 250mg/kg body weight and the effects were compared with the standard, indomethacin (10mg/kg body weight). It was obtained by exhibited highest anti-inflammatory activity at the dose 250mg/kg. The extract doses at 150 and 250mg/kg body weight) significantly prevented the increase in volume of paw edema.

Traditional uses of Ecliptae herba (EH) include bone strengthening. Ecliptae herba may have antiosteoporotic properties, according to accumulating data. The purpose of this research was to see how aqueous Ecliptae herba extract (EHE) affected diabetic osteoporosis. The effect of Ecliptae herba extract on blood glucose, HBA1C levels, and bone mineral density in rats was studied using a rat model. Twenty four male Sprague Dawley rats (n=6) were split into four groups: Saline was administered to normal control rats (NC), diabetic control rats (DC), diabetic rats were given 1000mg/kg body weight of metformin (MET) and1000mg/kg body weight of Ecliptae herba extractrespectively. When compared to normal controls, Ecliptae herba extract therapy significantly enhanced bone mass. This implies that Ecliptae herba extract might be developed as an alternative treatment for diabetes induced osteoporosis.

Objective(s):To compare and evaluate the Antimicrobial Efficacy of Different Light Activated Antimicrobial Agents (LAAAs) like citrus aurantifolia and Toluidine blue O against E. Faecalis within the root canal. Materials and Methods: 45 single rooted human permanent teeth, were used in this study. The teeth specimens were sectioned to obtain a standardized tooth length of 14 millimetre (mm). The root canals were cleaned and shaped using crown down technique with Protaper instruments (F3). Enterococcus faecalis (strain ATCC 29212) grown in tryptic soya broth (TSB) was inoculated in all the samples using a micropipette. Three experimental groups were formed of 15 samples each. Group I: Positive control. Group II (n=15): PDT with citrus aurantifolia oil. Group III (n=15): PDT with Toluidine Blue O. The optical fiber connected to diode laser was used to disinfect the canal. The shavings of the root canals were extracted using saline and 25 H-file, after which they were incubated in tryptic soya broth agar plates at 37^oC for 24hrs. The agar plates were analyzed for colony forming units (CFU) of Enterococccus faecalis in all the groups using a Digital colony counter and were subjected to statistical analysis using one way Analysis of Variance test (ANOVA). Pair-wise comparison was done using Post Hoc multiple comparison (Tukey) test. Results: The results of the study showed that, citrus aurantifolia showed antimicrobial effect when used as a photosensitizing agent. However TBO showed the best results followed essential oil of citrus aurantifolia against E. Faecalis. Conclusions: Within the limitations of the study, it was concluded that citrus aurantifolia has effective photosensitizing antimicrobialproperty and can be used as a potential LAAA.